Journal: Skeletal Muscle

Article Title: Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures

doi: 10.1186/s13395-016-0085-7

Figure Lengend Snippet: GRMD-myostatin status

Article Snippet: Quantification of myostatin was performed against recombinantly produced myostatin protein (R&D Systems, Inc. Minneapolis, MN, USA; catalog number 788-G8).

Techniques:

Journal: Skeletal Muscle

Article Title: Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures

doi: 10.1186/s13395-016-0085-7

Figure Lengend Snippet: Differences between GRMD ( Mstn +/+ ) and GRippet ( Mstn +/− ) dogs (significant ( p < 0.05) and trending ( p < 0.2))

Article Snippet: Quantification of myostatin was performed against recombinantly produced myostatin protein (R&D Systems, Inc. Minneapolis, MN, USA; catalog number 788-G8).

Techniques: Magnetic Resonance Imaging

Journal: Skeletal Muscle

Article Title: Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures

doi: 10.1186/s13395-016-0085-7

Figure Lengend Snippet: Myostatin mRNA levels in control, GRMD, and GRippet dogs. Values were significantly reduced in the CS of GRMD and GRippet dogs, compared to controls ( p < 0.01 for both). A less pronounced reduction was seen in the VL of GRMD and GRippet dogs ( p < 0.05 for both) versus controls. Values for the LDE and LHG did not differ among the three groups. No differences were seen between GRMD and GRippet dogs. All measurements are given in relation to HPRT values, which were normalized to 1

Article Snippet: Quantification of myostatin was performed against recombinantly produced myostatin protein (R&D Systems, Inc. Minneapolis, MN, USA; catalog number 788-G8).

Techniques: Control

Journal: Skeletal Muscle

Article Title: Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures

doi: 10.1186/s13395-016-0085-7

Figure Lengend Snippet: Western blots of ActRIIB levels normalized to β-actin. Blots from muscles of the two myostatin wild-type ( Racer and Endora ), three GRMD ( Flash , Samantha , and Hagatha ), and four GRippet ( Dash , Abner , Derwood , and Tabitha ) dogs are shown. Levels are upregulated in the dystrophic dogs compared to controls, but there is no consistent difference among individual muscles or between the GRMD and GRippet dogs. Levels are expressed in arbitrary units

Article Snippet: Quantification of myostatin was performed against recombinantly produced myostatin protein (R&D Systems, Inc. Minneapolis, MN, USA; catalog number 788-G8).

Techniques: Western Blot, Muscles